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The effects of Ca2+/calmodulin on the ATPase activity of the myosin subfragment 1 (S1) from rabbit cardiac myosin were investigated. In the presence of both 0.5 mM Ca2+ and 0.3 mM calmodulin, the ATPase activity was strongly activated (maximum approximately 3-fold at saturating [ATP]). S1(1-254) was found to have a low affinity for calmodulin (Kd approximately 30 microM) and a Ca(2+)-dependent calmodulin-binding capacity (KD approximately 1 microM) comparable with that of intact cardiac myosin. The binding of calmodulin to S1(1-254) was highly covalent; the reaction yielded approximately 0.4 mol calmodulin/mol of the S1 heavy chain. The addition of up to 0.8 mM bis(sulfosuccin
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